The Influence of Gamma Radiation on Different Gelatin Nanofibers and Gelatins.

Gelatin nanofibers are known as wound-healing biomaterials due to their high biocompatible, biodegradable, and non-antigenic properties compared to synthetic-polymer-fabricated nanofibers. The influence of gamma radiation doses on the structure of gelatin nanofiber dressings compared to gelatin of their origin is little known, although it is very important for the production of stable bioactive products. Different-origin gelatins were extracted from bovine and donkey hides, rabbit skins, and fish scales and used for fabrication of nanofibers through electrospinning of gelatin solutions in acetic acid. Nanofibers with sizes ranging from 73.50 nm to 230.46 nm were successfully prepared, thus showing the potential of different-origin gelatin by-products valorization as a lower-cost alternative to native collagen. The gelatin nanofibers together with their origin gelatins were treated with 10, 20, and 25 kGy gamma radiation doses and investigated for their structural stability through chemiluminescence and FTIR spectroscopy. Chemiluminescence analysis showed a stable behavior of gelatin nanofibers and gelatins up to 200 °C and increased chemiluminescent emission intensities for nanofibers treated with gamma radiation, at temperatures above 200 °C, compared to irradiated gelatins and non-irradiated nanofibers and gelatins. The electron paramagnetic (EPR) signals of DMPO adduct allowed for the identification of long-life HO● radicals only for bovine and donkey gelatin nanofibers treated with a 20 kGy gamma radiation dose. Microbial contamination with aerobic microorganisms, yeasts, filamentous fungi, Staphylococcus aureus, Escherichia coli, and Candida albicans of gelatin nanofibers treated with 10 kGy gamma radiation was under the limits required for pharmaceutical and topic formulations. Minor shifts of FTIR bands were observed at irradiation, indicating the preservation of secondary structure and stable properties of different-origin gelatin nanofibers.

Assessing Microplastics and Nanoparticles in the Surface Seawater of Venice Lagoon-Part I: Methodology of Research.

Microplastics (MPs) and nanoplastics (NPs) both represent significant concerns in environmental sciences. This paper aims to develop a convenient and efficient methodology for the detection and measurement of MPs and nanoparticles from surface seawater and to apply it to the water samples collected from the UNESCO site of Venice and its lagoon, more precisely in the Venice-Lido Port Inlet, Grand Canal under Rialto Bridge, and Saint Marc basin. In this study, MPs were analyzed through optical microscopy for their relative abundance and characterized based on their color, shape, and size classes, while the concentration and the mean of nanoparticles were estimated via the Nanoparticle Tracking Analysis technique. Bulk seawater sampling, combined with filtration through a cascade of stainless-steel sieves and subsequent digestion, facilitates the detection of MPs of relatively small sizes (size classes distribution: >1 mm, 1000-250 µm, 250-125 µm, 125-90 µm, and 90-32 µm), similar to the size of MPs ingested by marine invertebrates and fishes. A protocol for minimizing interference from non-plastic nanoparticles through evaporation, digestion, and filtration processes was proposed to enrich the sample for NPs. The findings contribute to the understanding of the extent and characteristics of MPs and nanoparticle pollution in the Venice Lagoon seawater, highlighting the potential environmental risks associated with these pollutants and the need for coordinated approaches to mitigate them. This article is based on scientific research carried out within the framework of the H2020 In-No-Plastic-Innovative approaches towards prevention, removal and reuse of marine plastic litter project (G.A. ID no. 101000612).

Fish Scale Gelatin Nanofibers with Helichrysum italicum and Lavandula latifolia Essential Oils for Bioactive Wound-Healing Dressings.

Essential oils are valuable alternatives to synthetic antibiotics that have the potential to avoid the pathogen resistance side effects generated by leather. Helichrysum italicum and Lavandula latifolia essential oils combined with fish scale gelatin were electrospun using a coaxial technique to design new bioactive materials for skin wound dressings fabrication. Fish scale gelatins were extracted from carp fish scales using two variants of the same method, with and without ethylenediaminetetraacetic acid (EDTA). Both variants showed very good electrospinning properties when dissolved in acetic acid solvent. Fish scale gelatin nanofibers with Helichrysum italicum and Lavandula latifolia essential oil emulsions ensured low microbial load (under 100 CFU/g of total number of aerobic microorganisms and total number of yeasts and filamentous fungi) and the absence of Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 10536, and Candida albicans ATCC 1023 as compared to fish scale gelatin without essential oils, which recommends them for pharmaceutical or topical applications. A scratch-test performed on human dermal fibroblasts proved that the biomaterials contributing to the wound healing process included fish scale gelatin nanofibers without EDTA (0.5% and 1%), fish scale gelatin nanofibers without EDTA and Lavandula latifolia essential oil emulsion (1%), fish scale gelatin nanofibers with EDTA (0.6%), and fish scale gelatin nanofibers with EDTA with Helichrysum italicum essential oil emulsion (1% and 2%).

Myopia & painful muscle form of temporomandibular disorders: connections between vision, masticatory and cervical muscles activity and sensitivity and sleep quality.

The main aim of this study is to evaluate the effects of painful muscle form of temporomandibular disorders and myopia on the connections between the visual organ, the bioelectrical activity and sensitivity of the masticatory and cervical muscles, and sleep quality. Subjects were divided into 4 groups (Myopia & TMDs, Myopia (Without TMDs), Emmetropic & TMDs and Emmetropic (Without TMDs)). The study was conducted in the following order of assessment: examination for temporomandibular disorders, assessment of the muscle activity by electromyograph, pressure pain thresholds examination, ophthalmic examination and completion of the Pittsburgh Sleep Quality Index. It was observed that the Myopia & TMDs group had higher muscle tenderness, higher resting and lower functional muscle bioelectrical activity. The visual organ is clinical related to the masticatory and cervical muscles. TMDs and myopia alter masticatory and cervical muscle activity. The thickness of the choroid in people with myopia is related to muscle tenderness. TMDs and myopia impair sleep quality. It is recommended to determine the number of people with refractive error and its magnitude in the sEMG study in order to be able to replicate the research methodology.

ZnO nanostructured matrix as nexus catalysts for the removal of emerging pollutants.

Water pollution stands as a pressing global environmental concern, elevating the significance of innovative, dependable, and sustainable solutions. This study represents an extensive review of the use of photocatalytic zinc oxide nanoparticles (ZnO NPs) for the removal of emerging pollutants from water and wastewater. The study examines ZnO NPs' different preparation methods, including physical, chemical, and green synthesis, and emphasizes on advantages, disadvantages, preparation factors, and investigation methods for the structural and morphological properties. ZnO NPs demonstrate remarkable properties as photocatalysts; however, their small dimensions pose an issue, leading to potential post-use environmental losses. A strategy to overcome this challenge is scaling up ZnO NP matrices for enhanced stability and efficiency. The paper introduces novel ZnO NP composites, by incorporating supports like carbon and clay that serve as photocatalysts in the removal of emerging pollutants from water and wastewater. In essence, this research underscores the urgency of finding innovative, efficient, and eco-friendly solutions for the removal of emerging pollutants from wastewater and highlights the high removal efficiencies obtained when using ZnO NPs obtained from green synthesis as a photocatalyst. Future research should be developed on the cost-benefit analysis regarding the preparation methods, treatment processes, and value-added product regeneration efficiency.

Insights into Anthropogenic Micro- and Nanoplastic Accumulation in Drinking Water Sources and Their Potential Effects on Human Health.

Anthropogenic microplastics (MPs) and nanoplastics (NPs) are ubiquitous pollutants found in aquatic, food, soil and air environments. Recently, drinking water for human consumption has been considered a significant pathway for ingestion of such plastic pollutants. Most of the analytical methods developed for detection and identification of MPs have been established for particles with sizes > 10 µm, but new analytical approaches are required to identify NPs below 1 µm. This review aims to evaluate the most recent information on the release of MPs and NPs in water sources intended for human consumption, specifically tap water and commercial bottled water. The potential effects on human health of dermal exposure, inhalation, and ingestion of these particles were examined. Emerging technologies used to remove MPs and/or NPs from drinking water sources and their advantages and limitations were also assessed. The main findings showed that the MPs with sizes > 10 µm were completely removed from drinking water treatment plants (DWTPs). The smallest NP identified using pyrolysis-gas chromatography-mass spectrometry (Pyr-GC/MS) had a diameter of 58 nm. Contamination with MPs/NPs can occur during the distribution of tap water to consumers, as well as when opening and closing screw caps of bottled water or when using recycled plastic or glass bottles for drinking water. In conclusion, this comprehensive study emphasizes the importance of a unified approach to detect MPs and NPs in drinking water, as well as raising the awareness of regulators, policymakers and the public about the impact of these pollutants, which pose a human health risk.

Correlation between refractive error, muscle thickness, and bioelectrical activity of selected masticatory muscles.

OBJECTIVE: The aim of this study was to analyze the correlation between the refractive error, muscle thickness, and the bioelectrical activity of selected masticatory and neck muscles in subjects with myopia. METHODS: For bioelectrical activity analysis within the masticatory muscles, an 8-channel BioEMG III electromyograph was used. An M-Turbo ultrasound machine was used to analyze the masticatory and neck muscle thickness. RESULTS: Statistical analysis showed significant positive correlations between masseter muscle thickness at rest on the right side. Statistical analysis showed negative correlations between the digastric muscle and Activity Index within masticatory muscles at rest on the left side when the eyes were closed. CONCLUSIONS: As refractive error increases in myopic subjects, the resting tension on the temporal muscles increases, an increase in masseter muscle thickness is noted, and a decrease in bioelectrical activity on the digastric muscle at rest.

The Influence of Visual Input on Electromyographic Activity and Patterns of Masticatory and Cervical Spine Muscles in Emmetropic Caucasian Subjects by Gender.

(1) Background: The objective of the research was to analyze the change of visual input on electromyographic activity and patterns of masticatory and cervical spine muscles in emmetropic Caucasian subjects by gender. Supposedly, visual input should not influence activity and electromyographic patterns of masticatory and cervical spine muscles in emmetropic Caucasian subjects by gender. (2) Methods: After applying the inclusion criteria, 50 emmetropic Caucasian subjects were included in the study. Four muscle pairs were analyzed: the temporalis muscle (TA), the masseter muscle (MM), the digastric muscle (DA), and the sternocleidomastoid muscle (SCM), during resting and functional activity. (3) Results: It was observed that there were no significant statistical differences in activity and bioelectrical patterns between open and closed eyes in women and men, with the exception of clenching on dental cotton rollers in DA-left and DA mean between tests in women. The observed statistical results had a small effect size, successively equal to 0.32 and 0.29. (4) Conclusions: Changes in the influence of visual input do not affect electromyographic activity and patterns of masticatory and cervical spine muscles in emmetropic Caucasian women and men.

Exploratory Study on Central Sensitization and Bioelectrical Activity of the Selected Masticatory Muscles in Subjects with Myopia.

BACKGROUND: Myopia is one of the most common eye disorders involving abnormal focusing of light rays. The studies recognize the association between the stomatognathic and visual systems. This compound may have a neurological basis associated with disorders such as central sensitization. The main aim of this study was to evaluate the influence of central sensitization on the bioelectrical activity of selected muscles of the masticatory organ in subjects with myopia. METHODS: Selected masticatory and cervical spine muscles were analyzed using an eight-channel BioEMG III electromyograph. Central sensitization was analyzed using the central sensitization inventory. RESULTS: Statistical analysis revealed significantly higher scores on the central sensitization inventory in subjects with axial myopia compared to subjects without refractive error. Repeated positive correlations were observed in the sternocleidomastoid muscle activity and negative correlations in the digastric muscle activity during open and closed eyes in myopic subjects. CONCLUSIONS: Subjects with myopia have an increased score in the central sensitization inventory. The increase in the central sensitization inventory score is connected with the changes within the electromyographic activity of the masticatory and neck muscles. The effect of central sensitization on masticatory muscle activity in myopic subjects requires further study.

The Incidence and Severity of Myopia in the Population of Medical Students and Its Dependence on Various Demographic Factors and Vision Hygiene Habits.

(1) Background: Myopia is one of the leading causes of visual impairment. Visual work and usage of electronic devices are known risk factors of myopia. Many education systems were forced to apply online and hybrid teaching methods, to reduce the number of new cases of COVID-19. Medical students are a population well-known for intense visual work in the form of learning; (2) Methods: Visual acuity and refractive error were measured in the population of medical students. Participants also filled out the survey that included their population characteristic and their habits related to the hygiene of vision; (3) Results: We found a correlation between the age of the first diagnosis of myopia and current values of refractive error. The majority of participants believe that the COVID-19 pandemic had an impact on the health of their vision. Among methods of studying, usage of the computer screen was less preferred by myopic students; (4) Conclusions: In the population of medical school students in Eastern Poland, visual acuity was lower than 1.0 in 232 (52.97%) in the right eye and 234 (53.42%) in the left eye. Early recognition of refractive error has influenced its current values. Among methods of studying, usage of the computer screen was less preferred by myopic students. More population-based studies should focus on the impact of the COVID-19 pandemic on the health of vision.

Antioxidant and Antimicrobial Properties of Hydrolysed Collagen Nanofibers Loaded with Ginger Essential Oil.

Hydrolysed collagen obtained from bovine leather by-products were loaded with ginger essential oil and processed by the electrospinning technique for obtaining bioactive nanofibers. Particle size measurements of hydrolysed collagen, GC-MS analysis of ginger essential oil (EO), and structural and SEM examinations of collagen nanofibers loaded with ginger essential oil collected on waxed paper, cotton, and leather supports were performed. Antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli and antifungal activity against Candida albicans were also determined. Data show that the hydrolysed collagen nanofibers loaded with ginger EO can be used in the medical, pharmaceutical, cosmetic, or niche fields.

Structural Insights into LDPE/UHMWPE Blends Processed by γ-Irradiation.

Ultra-high-molecular-weight polyethylene (UHMWPE) matrices containing low-density polyethylene (LDPE), hydroxyapatite (HAp) as filler, and rosemary extract (RM) as stabilizer were investigated for their qualification for long-term applications. The significant contributions of the blend components were analyzed, and variations in mechanical properties, oxidation strength, thermal behavior, crystallinity, and wettability were discussed. SEM images of microstructural peculiarities completed the introspective survey. The stability improvement due to the presence of both additives was an increase in the total degradation period of 67% in comparison with an unmodified HDPE/UHMWPE blend when the materials were subjected to a 50 kGy γ-dose. There was growth in activation energies from 121 kJ mol-1 to 139 kJ mol-1 when HAp and rosemary extract delayed oxidation. The exposure of samples to the action of γ-rays was found to be a proper procedure for accomplishing accelerated oxidative degradation. The presence of rosemary extract and HAp powder significantly increased the thermal and oxidation resistances. The calculation of material lifetimes at various temperatures provided meaningful information on the wearability and integrity of the inspected composites.

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV))/Bacterial Cellulose (BC) Biocomposites for Potential Use in Biomedical Applications.

The aim of this study was to obtain biocomposites consisting of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), bacterial cellulose (BC) and α-tocopherol by a melt processing technique for potential use in biomedical applications. The melt processing and roughness of biocomposites were evaluated and compared to sample without BC. The degradation rate of PHBV/BC biocomposites was measured in phosphate buffer saline (PBS) by determining the mass variation and evidencing of thermal and structural changes by differential scanning calorimetry (DSC) and attenuated total reflectance-Fourier transformed infrared spectrometry (ATR-FTIR). The cell viability, cell morphology, cell cycle distribution and total collagen content were investigated on murine NCTC fibroblasts. Overall, the adding of BC to polyester matrix led to an adequate melt processing of biocomposites and increased surface roughness and cytocompatibility, allowing the cells to secrete the extracellular matrix (collagen) and stimulate cell proliferation. Results showed that the PHBV/BC biocomposites were favorable for long-term degradation and could be used for the design of medical devices with controlled degradability.

Antimicrobial Active Packaging Containing Nisin for Preservation of Products of Animal Origin: An Overview.

The preservation of food represents one of the greatest challenges in the food industry. Active packaging materials are obtained through the incorporation of antimicrobial and/or antioxidant compounds in order to improve their functionality. Further, these materials are used for food packaging applications for shelf-life extension and fulfilling consumer demands for minimal processed foods with great quality and safety. The incorporation of antimicrobial peptides, such as nisin, has been studied lately, with a great interest applied to the food industry. Antimicrobials can be incorporated in various matrices such as nanofibers, nanoemulsions, nanoliposomes, or nanoparticles, which are further used for packaging. Despite the widespread application of nisin as an antimicrobial by directly incorporating it into various foods, the use of nisin by incorporating it into food packaging materials is researched at a much smaller scale. The researchers in this field are still in full development, being specific to the type of product studied. The purpose of this study was to present recent results obtained as a result of using nisin as an antimicrobial agent in food packaging materials, with a focus on applications on products of animal origin. The findings showed that nisin incorporated in packaging materials led to a significant reduction in the bacterial load (the total viable count or inoculated strains), maintained product attributes (physical, chemical, and sensorial), and prolonged their shelf-life.

Ferrous Industrial Wastes-Valuable Resources for Water and Wastewater Decontamination.

Ferrous waste by-products from the metallurgical industry have a high potential for valorization in the context of the circular economy, and can be converted to value-added products used in environmental remediation. This research reviews the latest data available in the literature with a focus on: (i) sources from which these types of iron-based wastes originate; (ii) the types of ferrous compounds that result from different industries; (iii) the different methods (with respect to the circular economy) used to convert them into products applied in water and wastewater decontamination; (iv) the harmful effects ferrous wastes can have on the environment and human health; and (v) the future perspectives for these types of waste.

Walnut Shell Biowaste Valorization via HTC Process for the Removal of Some Emerging Pharmaceutical Pollutants from Aqueous Solutions.

This research emphasizes the performance of some eco-friendly carbon materials as hydrochars (HC) obtained by the hydrothermal carbonization (HTC) process applied to walnut shell (WS) biowaste. These materials display promising properties that can be used for environmental applications such as emerging pharmaceutical pollutant retention from water sources. Thus, three hydrochars coded HCWS1, HCWS2, and HCWS3 were obtained using a dynamic autoclave in specific conditions-temperature of 220 °C, autogenous pressure, 1:10 biomass-water weight ratio-and for three different reaction times, 1 h, 6 h, and 12 h. The HCWSs were characterized by means of ATR-FTIR and SEM-EDS analyses and tested as possible adsorbents to assess the removal efficiencies of some emerging pharmaceutical pollutants (paracetamol and methylene blue) by UV-VIS spectrophotometry. Kinetic and adsorption studies were carried out. The best results were obtained for the HCWS3 hydrochar. Further perspectives include an activation step of the hydrochars and their testing on other emerging pharmaceutical pollutants.

Valorization of Polypropylene Waste in the Production of New Materials with Adequate Mechanical and Thermal Properties for Environmental Protection.

Innovative composites based on polypropylene waste impurified cu HDPE (PPW) combined with two thermoplastic block-copolymers, namely styrene-butadiene-styrene (SBSBC) and styrene-isoprene-styrene (SISBC) block-copolymers, and up to 10 wt% nano-clay, were obtained by melt blending. SBSBC and SISBC with almost the same content of polystyrene (30 wt%) were synthesized by anionic sequential polymerization and used as compatibilizers for PPW. Optical microscopy evaluation of the PPW composites showed that the n-clay was encapsulated into the elastomer. Addition of n-clay, together with SBSBC or SISBC, increased the interphase surface of the components in the PPW composites and enhanced the superficial area/volume ratio, which led to a recycled material with improved performance. The data resulting from differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), mechanical evaluation, and dynamic mechanical analysis (DMA) revealed that PPW reinforcement with n-clay and styrene-diene block-copolymers allows the obtaining of composites with favorable mechanical and thermal properties, and excellent impact strength for potential engineering applications.

Evaluation of Natural and Modified Castor Oil Incorporation on the Melt Processing and Physico-Chemical Properties of Polylactic Acid.

Bio-based plasticizers derived from renewable resources represent a sustainable replacement for petrochemical-based plasticizers. Vegetable oils are widely available, non-toxic and biodegradable, resistant to evaporation, mostly colorless and stable to light and heat, and are a suitable alternative for phthalate plasticizers. Plasticized poly(lactic acid) (PLA) materials containing 5 wt%, 10 wt%, 15 wt% and 20 wt% natural castor oil (R) were prepared by melt blending to improve the ductility of PLA. Three castor oil adducts with maleic anhydride (MA), methyl nadic anhydride (methyl-5-norbornene-2,3-dicarboxylic anhydride) (NA) and hexahydro-4-methylphthalic anhydride (HA), previously synthesized, were incorporated in a concentration of 15 wt% each in PLA and compared with PLA plasticized with natural R. The physico-chemical properties of PLA/R blends were investigated by means of processability, chemical structure, surface wettability, mechanical, rheological and thermal characteristics. The addition of natural and modified R significantly improved the melt processing by decreasing the melt viscosity by ~95%, increased the surface hydrophobicity, enhanced the flexibility by ~14 times in the case of PLA/20R blend and ~11 times in the case of PLA/15R-MA blend as compared with neat PLA. The TG/DTG results showed that the natural R used up to 20 wt% could significantly improve the thermal stability of PLA, similar to the maleic anhydride-modified R. Based on the obtained results, up to 20 wt% natural R and 15 wt% MA-, HA- or NA-modified R might be used as environmentally friendly plasticizers that can improve the overall properties of PLA, depending on the intended food packaging applications.

Bioactivity and Thermal Stability of Collagen-Chitosan Containing Lemongrass Essential Oil for Potential Medical Applications.

Bioactive collagen-chitosan-lemongrass (COL-CS-LG) membranes were prepared by casting method and analyzed for potential biomedical applications. For COL-CS-LG membranes, LG essential oil release, antioxidant properties, in vitro cytotoxicity and antimicrobial assessments were conducted, as well as free radical determination after gamma irradiation by chemiluminescence, and structural characteristics analysis through Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR) and Differential Scanning Calorimetry (DSC). The evaluation of non-isothermal chemiluminescence after gamma radiation exposure to COL-CS-LG membranes revealed a slowing down of the oxidation process at temperatures exceeding 200 °C, in correlation with antioxidant activity. Antimicrobial properties and minimum inhibitory concentrations were found to be in correlation with cytotoxicity limits, offering the optimum composition for designing new biomaterials.

The Axial Length of the Eyeball and Bioelectrical Activity of Masticatory and Neck Muscles: A Preliminary Report.

Objective: The present study aimed to evaluate the correlation of eye length and bioelectric activity of temporalis, masseter, digastric, and sternocleidomastoid muscles in women with myopia compared to healthy women. Methods: Based on the exclusion and inclusion criteria, 42 women aged 24 years (±2 years) were eligible for the study. Two equally sized groups with myopic (n = 21) and emmetropic healthy subjects (n = 21) were formed. An electromyographic study of the examined muscles was performed in four conditions: at rest, during maximal voluntary clenching in the intercuspal position, during maximal voluntary clenching on dental cotton rollers, and during maximal mouth opening using BioEMG III (BioResearch Associates, Inc. Milwaukee, WI, USA). The IOL Master 500 (Carl Zeiss Meditec, Jena, Germany) was used to examine the eyeball length. Statistical analysis showed significant positive correlations during mouth opening in both groups with open and closed eyes. Results: A greater number of correlations between the analyzed variables was observed in emmetropic women. In almost all cases, the longer axial eye length was associated with an increase in the bioelectrical activity of the analyzed muscles. Significant correlations were most often observed within the masseter and digastric muscles during the maximum mouth opening and at rest. Conclusion: There is a relationship between the bioelectrical activity of the masticatory muscles and the axial length of the eyeball on the same side.